DNAWorks 3.2.4

DNAWorks takes as input nucleotide and/or protein sequences, codon information, and other variables, and attempts to optimize a synthetic gene. It then outputs the gene with a variety of histograms and metrics for judging the probability of success for generating the gene by PCR. It also outputs the oligonucleotide sequences required for PCR synthesis of the synthetic gene.

Codon Frequency Table

Please choose a standard organism:

Or enter custom codon frequency table below:

Parameters:

Annealing Temperature: °C          to °C

Oligo Length: nt          to nt          Random

Codon Frequency Threshold: % Random Strict Scored

Oligonucleotide Concentration: M

Na+/K+ Concentration: M

Mg2+ Concentration: M

Number of Solutions:

TBIO Model

No Gaps in Assembly

Restriction Site Screen (Optional):

Sites selected will be excluded from the protein coding region of the synthetic gene.


Non-Degenerate Sites:





















































































































Degenerate Sites:































































Custom Site Screen (Optional):

Input your own site to be excluded from the protein coding region(s) of the synthetic gene.


Weights (Optional):
TWT: CWT: RWT: MWT: GWT: AWT: LWT: PWT: FWT:
Sequence(s):

Protein Nucleotide          Reverse Sequence Fix Sequence in Gap

Upload Excel File:
Or Input Sequence below: